Two ω-3 FADs Are Associated with Peach Fruit Volatile Formation.
نویسندگان
چکیده
Aroma-related volatiles, together with sugars and acids, play an important role in determining fruit flavor quality. Characteristic volatiles of peach fruit are mainly derived from fatty acids such as linoleic acid (18:2) and linolenic acid (18:3). In the present study, six genes encoding fatty acid desaturases (FAD) were cloned, including two ω-6 FAD genes (PpFAD2, PpFAD6) and four ω-3 FAD genes (PpFAD3-1, PpFAD3-2, PpFAD7 and PpFAD8). Heterologous expression of peach FADs in tobacco plants showed that PpFAD3-1, and PpFAD3-2 significantly reduced contents of 18:2, and accumulated significant higher levels of 18:3. In the case of volatiles, transgenic plants produced lower concentrations of hexanal and higher levels of (E)-2-hexenal. Consequently, the ratio of the (E)-2-hexenal and hexanal was about 5- and 3-fold higher than that of wild type (WT) in PpFAD3-1 and PpFAD3-2 transformants, respectively. No significant changes in volatile profiles were observed in transgenic plants overexpressing the four other peach FAD genes. Real-time quantitative polymerase chain reaction (qPCR) analysis showed that ripe fruit had high PpFAD3-1 and low PpFAD3-2 transcript levels. In contrast, high PpFAD3-2 and low PpFAD3-1 transcript levels were observed in young fruit. These results indicate a temporal regulation of these two ω-3 FADs during development and ripening, influencing peach fruit volatile formation.
منابع مشابه
A Non-Targeted Approach Unravels the Volatile Network in Peach Fruit
Volatile compounds represent an important part of the plant metabolome and are of particular agronomic and biological interest due to their contribution to fruit aroma and flavor and therefore to fruit quality. By using a non-targeted approach based on HS-SPME-GC-MS, the volatile-compound complement of peach fruit was described. A total of 110 volatile compounds (including alcohols, ketones, al...
متن کاملCharacterization and expression analysis of FRUITFULL- and SHATTERPROOF-like genes from peach (Prunus persica) and their role in split-pit formation.
The fruit canning industry processes large quantities of the clingstone varieties of peach (Prunus persica L. Batch). The occurrence of split-pit formation--the opening of the pit and sometimes splitting of the fruit--causes deterioration of canned fruit quality. The frequency of split-pit formation is influenced by genetic and environmental factors. To increase understanding of the molecular m...
متن کاملEffect of Pre-Harvest Salicylic Acid and Iron Treatments on Postharvest Quality of Peach Fruits
Peach is a highly corrosive fruit with a short shelf life (less than 7 days at room temperature)susceptible to diseases, pathogens and physical damage. The storage or marketable life ofhorticultural crops can be extended by various treatments applied to them after and/or beforeharvesting. Fruits are usually treated with a range of materials [(e.g. salicylic acid (SA)] to...
متن کاملComparative Transcriptome and Microscopy Analyses Provide Insights into Flat Shape Formation in Peach (Prunus persica)
Fruit shape is an important external characteristic that consumers use to select preferred fruit cultivars. In peach, the flat fruit cultivars have become more and more popular worldwide. Genetic markers closely linking to the flat fruit trait have been identified and are useful for marker-assisted breeding. However, the cellular and genetic mechanisms underpinning flat fruit formation are stil...
متن کاملThe Use of Non-destructive Methods to Analyse Fruit Quality
This article is divided into three parts. The first part, the most important one, deals with spectroscopic methods. Different regions of the electromagnetic spectrum are useful for fruit quality characterisation, in particular the visible and near-infrared regions. Concerning the visible one, studies have been carried out on apple, apricot, cherry, mango, peach, red table grape and tomato, when...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- International journal of molecular sciences
دوره 17 4 شماره
صفحات -
تاریخ انتشار 2016