Preferential binding of a chick brain tau isoform to microtubules assembled in the presence of aluminium fluoride.
نویسنده
چکیده
The concentration of [3H]DHA was 1.0 n M . Non-specific binding was defined by the curve-fitting program and it was determined as 29% of total [”IDHA binding. The competition curve fit best to a two-site model (f.’<O.0025), with K , = 4 f 2 nM and K L = 3 f 2 ,UM ( m e a n s + s ~ ~ of three separate competition experiments which were pooled). These values were obtained by setting the affinity of [”IDHA for the two sites at 1.8 n M .
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عنوان ژورنال:
- Biochemical Society transactions
دوره 18 3 شماره
صفحات -
تاریخ انتشار 1990