Nucleotide sequence of the human parainfluenza virus 3 matrix protein gene.
نویسندگان
چکیده
The nucleotide sequence of the matrix (M) gene of human parainfluenza virus 3 (HPIV3), strain 47885, was determined using overlapping cDNA clones derived from mRNA and genomic RNA. The sequence is presented as DNA in the mRNA sense beginning with the first nucleotide of M mRNA. The HPIV3 M gene is 1150 nucleotides long, excluding 5 3'terminal A residues. A single long open reading frame begins at nucleotide 33 and encodes a polypeptide of 353 amino acids (molecular weight 39499). The HPIV3 M protein is basic (net charge + 20.5 at pH 7). Many arginine and lysine residues are conserved among pararaysovirus M proteins (1--1). Two slightly hydrophobic stretches sre four.i (amino acids 173-208 and 264-294); the second within a region, with a predicted secondary structure common among paramyxovirus M proteins, believed to interact with but not span the envelope (4-6). The HPIV3 M protein exhibits considerable homology with the M protein of Sendai virus Cv-62%) but less homology with the M proteins of measles virus K30%), and NDV (018%).
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عنوان ژورنال:
- Nucleic acids research
دوره 15 7 شماره
صفحات -
تاریخ انتشار 1987