Intrabody expression in eukaryotic cells.
نویسندگان
چکیده
We describe procedures for intracellular expression of scFv in eukaryotic cells. Starting from a scFv gene cloned in a phage-display vector, we describe the cloning step into a mammalian expression vector, the transient transfection of a HeLa cell line, and the monitoring of intrabody expression by immunofluorescence staining and FACS analysis.
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عنوان ژورنال:
- Methods in molecular biology
دوره 562 شماره
صفحات -
تاریخ انتشار 2009