Studies in 2-acetylaminofluorene carcinogenesis. I. The intracellular distribution of nucleic acids and protein in rat liver.

Studies on the intracellular composition of livers from rats fed 2-acetylaminofluorene.

Price and his associates (17, 18, 19) found al terations in the amounts of protein, nucleic acids, and riboflavin in various fractions of the liver during the induction of liver tumors by a series of aminoazo dyes of widely different carcinogenic ac tivities. The changes induced by the more active carcinogens made the composition of the liver ap proach that of the tumors induced by the parent c...

Studies in 2-acetylaminofluorene carcinogenesis. II. The in vitro uptake of alanine-1-C14 by preneoplastic liver and hepatoma mitochondrial protein.

Zamecnik et al. (19-21) demonstrated that sur viving DAB1 hepatoma slices incorporate more radioactivity from amino acids, carbohydrates, and related intermediates into slice proteins than do normal rat liver slices. This observation sug gested the desirability of testing the in vitro activi ty of liver preparations during AAF1 administra tion, thereby providing comparable information on this c...

Covalent Binding of 2-Acetylaminofluorene, 2-Aminofluorene, and /V-Hydroxy-2-acetylaminofluorene to Rat Liver Nuclear DMA and Protein in Vivo and in Wfro1

Binding of the hepatocarcinogen 2-acetylaminofluorene (AAF) and two metabolites, 2-aminofluorene (AF) and W-hydroxy-2-acetylaminofluorene (N-OH-AAF), to the DNA and pro tein of rat hepatic nuclei was examined in vitro and in a cellfree system. Three and one-half hr following a single injection of each compound in equimolar amounts, DNA contained ap proximately 50% more of the compounds per mg t...

The Role of N-sulfation in the Promotion Phase of Carcinogenesis by N-hydroxy-2-acetylaminofluorene in Male Rat Liver

Persistent binding of 2-acetylaminofluorene to rat liver DNA in vivo and consideration of the mechanism of binding of N-hydroxy-2-acetylaminofluorene to rat liver nucleic acids.

Previous reports that radioactivity was bound to rat liver DNA in vivo after administration of 2-acetylaminofluorene (AAF)-9-14C were confirmed. In addition, administration of several other AAF derivatives, namely N-hydroxy-AAF-9 -14C, N-acetoxy-AAF-914C, and the glucuronide of N-hydroxyAAF-9)4C, led to the binding of higher levels of radioactivity to rat liver ribosomal RNA and DNA. Maximum le...