The replication protein of the Sulfolobus islandicus plasmid pRN1.
نویسنده
چکیده
The thermoacidophile crenarchaeote Sulfolobus ssp. is one of the best-studied Archaea. Cryptic and conjugative plasmids as well as viruses have been described for this genus. For the majority of the genetic elements only the genome sequence and the basic characteristics were determined. In contrast the fusellovirus SSV1 and the cryptic plasmid pRN1, which is the smallest known genetic element of the crenarchaeota, have been studied in more detail. The three gene products of the plasmid pRN1 have been characterized biochemically. The replication protein of the plasmid, a multifunctional enzyme, has a novel domain, termed prim/pol domain. This domain constitutes the first member of the DNA polymerase family E. Based on the biochemical characterization of the gene products a model of how pRN1 is replicated in vivo is proposed.
منابع مشابه
Complete nucleotide sequence of the Sulfolobus islandicus multicopy plasmid pRN1.
The complete sequence of the 5350-bp plasmid pRN1 from the crenarchaeote Sulfolobus islandicus has been determined. This plasmid is the first to be sequenced from this group of thermoacidophilic archaebacteria (Archaea) and its high copy number and wide host range make it a good candidate for a cloning vector. pRN1 contains several open reading frames, including one that spans over half the pla...
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The 5.4-kb and 6.9-kb plasmids pRN1 and pRN2 from the crenarchaeon Sulfolobus islandicus are name-giving for a small family of archaeal plasmids. Both plasmids have hitherto been supposed to be dependent on each other because they are always found together in their natural host. Here we demonstrate that each of the plasmids can stably propagate and replicate on its own independent of the other ...
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عنوان ژورنال:
- Biochemical Society transactions
دوره 32 Pt 2 شماره
صفحات -
تاریخ انتشار 2004