Red Cells Cybrd1 (duodenal cytochrome b) is not necessary for dietary iron absorption in mice Running Head: Targeted disruption of Cybrd1
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چکیده
Author Contributions HG designed and participated in the execution of all experiments described in this report and contributed to writing the manuscript. HG, CNS and VMS initially designed and prepared the Cybrd1 gene-targeting construct. MDF and ELG produced and characterized anti-Cybrd1 antiserum. JJ assisted with detection of Cybrd1 and Slc11a2 proteins in enterocytes. CD and SMG provided technical assistance. NCA guided the overall development of the research plan and contributed to writing the manuscript.
منابع مشابه
Cybrd1 (duodenal cytochrome b) is not necessary for dietary iron absorption in mice.
Mammalian nonheme iron absorption requires reduction of dietary iron for uptake by the divalent metal ion transport system in the intestine. This was thought to be mediated by duodenal cytochrome b (Cybrd1), a ferric reductase enzyme resident on the luminal surface of intestinal absorptive cells. To test its importance in vivo, we inactivated the murine Cybrd1 gene and assessed tissue iron stor...
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Duodenal cytochrome b (Dcytb) is a haem protein similar to the cytochrome b561 protein family. Dcytb is highly expressed in duodenal brush-border membrane and is implicated in dietary iron absorption by reducing dietary ferric iron to the ferrous form for transport via Nramp2/DCT1 (divalent-cation transporter 1)/DMT1 (divalent metal-transporter 1). The protein is expressed in other tissues and ...
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Iron and ascorbate are vital cellular constituents in mammalian systems. The bulk-requirement for iron is during erythropoiesis leading to the generation of hemoglobin-containing erythrocytes. Additionally; both iron and ascorbate are required as co-factors in numerous metabolic reactions. Iron homeostasis is controlled at the level of uptake; rather than excretion. Accumulating evidence strong...
متن کاملEffect of lipopolysaccharide and bleeding on the expression of intestinal proteins involved in iron and haem transport.
Haem carrier protein 1 (Hcpl) is a component of the haem-iron uptake pathway in the small intestine. Using quantitative real-time PCR, we examined the expression of Hcp1 and other intestinal iron-transporting proteins in male C57BL/6 mice with experimentally altered iron homeostasis. Intestinal Hcp1 mRNA content was not significantly changed by iron overload (600 mg/kg); however, it was increas...
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تاریخ انتشار 2005