"Turn on" and label-free core−shell Ag@SiO2 nanoparticles-based metal-enhanced fluorescent (MEF) aptasensor for Hg2+
نویسندگان
چکیده
A turn on and label-free fluorescent apasensor for Hg(2+) with high sensitivity and selectivity has been demonstrated in this report. Firstly, core-shell Ag@SiO2 nanoparticles (NPs) were synthetized as a Metal-Enhanced Fluorescent (MEF) substrate, T-rich DNA aptamers were immobilized on the surface of Ag@SiO2 NPs and thiazole orange (TO) was selected as fluorescent reporter. After Hg(2+) was added to the aptamer-Ag@SiO2 NPs and TO mixture buffer solution, the aptamer strand can bind Hg(2+) to form T-Hg(2+)-T complex with a hairpin structure which TO can insert into. When clamped by the nucleic acid bases, the fluorescence quanta yield of TO will be increased under laser excitation and emitted a fluorescence emission. Furthermore, the fluorescence emission can be amplified largely by the MEF effect of the Ag@SiO2 NPs. The whole experiment can be finished within 30 min and the limit of detection is 0.33 nM even with interference by high concentrations of other metal ions. Finally, the sensor was applied for detecting Hg(2+) in different real water samples with satisfying recoveries over 94%.
منابع مشابه
Corrigendum: “Turn on” and label-free core−shell Ag@SiO2 nanoparticles-based metal-enhanced fluorescent (MEF) aptasensor for Hg2+
متن کامل
Self-assembly of conjugated polymer-Ag@SiO2 hybrid fluorescent nanoparticles for application to cellular imaging.
A novel fluorescent nanoparticle was prepared via a simple self-assembly technique based on water-soluble conjugated polymers (CPs) and Ag@SiO(2) core-shell nanoparticles. Core-shell nanoparticles with silver NPs core show a unique property referred to as metal-enhanced fluorescence (MEF). In the present work, the cationic conjugated polymer poly[9,9'-bis(6''-(N,N,N-trimethylammonium)-hexyl) fl...
متن کاملCo-enhancement of fluorescence and singlet oxygen generation by silica-coated gold nanorods core-shell nanoparticle
Metal-enhanced fluorescence (MEF) as a newly recognized technology has been attracting considerable attention and is widely used in fluorescence-based technology. In this paper, we reported a novel distance-dependent MEF and metal-enhanced singlet oxygen generation phenomenon based on silica-coated gold nanorods (AuNRs@SiO2) core-shell structure with tetra-substituted carboxyl aluminum phthaloc...
متن کاملCore-shell Ag@SiO2@mSiO2 mesoporous nanocarriers for metal-enhanced fluorescence.
A novel mesoporous nanocarrier consisting of a silver core, a silica spacer with controlled thickness and a fluorophores-loaded mesoporous silica shell was fabricated for the metal-enhanced fluorescence (MEF) and Förster resonance energy transfer (FRET) effects.
متن کاملA Novel Electrochemical Aptamer Biosensor Based on DNAzyme Decorated Au@Ag Core-Shell Nanoparticles for Hg2+ Determination
As a kind of important water pollutant, heavy metal ions have a detrimental effect on the health of human. In this study, Au@Ag core-shell nanoparticles were synthesized by simple reduction of Ag around Au nanoparticles and functionalized by DNAzyme. Based on thymine-Hg-thymine binding mode, a sensitive aptamer biosensor was constructed by utilizing functionalized Au@Ag core-shell nanoparticles...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره 5 شماره
صفحات -
تاریخ انتشار 2015