Methanolic leaf exctract of Otostegia integrifolia Benth reduces blood glucose levels in diabetic, glucose loaded and normal rodents
نویسندگان
چکیده
BACKGROUND Although the leaves of Otostegia integrifolia traditionally claimed in Ethiopian folklore medicine for management of diabetes mellitus, it has not been validated. The aim of this study was therefore to investigate the antidiabetic activity of Otostegia integrifolia in rodents. METHODS Male rats or mice were randomly divided into five groups for diabetic, hypoglycemic and glucose tolerance test (OGTT) studies. In all models, group I received Tween 80, 2% v/v, Group II (GL5) treated with 5 mg/kg of glibenclamide and the remaining group: III, IV and V were given the plant extract at doses of 100 mg/kg 200 mg/kg and 400 mg/kg respectively. Blood glucose levels (BGL) were measured by collecting blood samples at different time points. Data was analyzed using one way ANOVA followed by Dunnet's post hoc test to carry out between and within group comparisons. P < 0.05 was considered as statistically significant. RESULTS Inter-group analysis revealed that O. integrifolia at 100 mg/kg and 200 mg/kg reduced the 4(th) hour fasting blood BGL significantly (p < 0.001) compared to the control group. The intra-group analysis result has shown O. integrifolia at 200 mg/kg produced a significant (p < 0.05) reduction in BGL at the 1(st), 2(nd), 3rd and 4th hours of post treatment compared to their respective initial levels. Moreover, in the hypoglycemic and OGTT models, O. integrifolia extract at 200 mg/kg, has shown a significant reduction in blood glucose levels compared to negative controls and across all time points. CONCLUSION The crude extract of O. integrifolia has shown significant antidiabetic, hypoglyceamic and oral glucose tolerating effects. The effective dose of the extract was found to be 200 mg/kg in time dependent manner.
منابع مشابه
اثر عصاره متانولی Otostegia persica بر سطح سرمیگلوکز و آنزیمهای شاخص عملکرد کبد در موشهای صحرایی نر دیابتی شده با استرپتوزوتوسین
The Effect of Methanolic Extract of Otostegia persica on Serum Levels of Glucose and Liver Function Enzymes in Streptozotocin -Induced Diabetic Male Rats M. Hedayati [1] , I. Pouraboli [2] , M. Mirtajaddini [3] Received: 15/06/10 Sent for Revision: 04/09/10 Received Revised Manuscript: 17/10/10 Accepted: 31/10/10 Background and Objectives : With respect to antioxidant effects of O...
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