Electron Microscopy of Biomolecules

“ for developing cryo - electron microscopy for the high - resolution structure determination of biomolecules in solution ” 2017 Nobel Prize in Chemistry

Dual-target function validation of single-particle selection from low-contrast cryo-electron micrographs

Weak-signal detection and single-particle selection from low-contrast micrographs of frozen hydrated biomolecules by cryo-electron microscopy (cryoEM) presents a practical challenge. Cryo-EM image contrast degrades as the size of biomolecules of structural interest decreases. When the image contrast falls into a range where the location or presence of single particles becomes ambiguous, a need ...

Evaluation of Antioxidant, Antibacterial and Photo catalytic Effect of Silver Nanoparticles from Methanolic Extract of Coleus Vettiveroids – an Endemic Species

Biosynthesis of metal nanoparticles using plant extract has received much attention due to its eco-friendly nature. The present study elucidates the green synthesize of Silver nanoparticles (AgNPs) from methanolic extract of Coleus Vettiveroids –an endemic species. The synthesis of AgNPs was confirmed by UV-visible spectrometry at 416 nm. Further, biosynthesized nanoparticles were characterized...

Demystifying EPR: A Rookie Guide to the Application of Electron Paramagnetic Resonance Spectroscopy on Biomolecules

Electron Paramagnetic Resonance (EPR) spectroscopy, also known as Electron Spin Resonance(ESR) especially among physicists, is a strong and versatile spectroscopic method forinvestigation of paramagnetic systems, i.e. systems like free radicals and most transition metalions, which have unpaired electrons. The sensitivity and selectivity of EPR are notable andintriguing as compared to other spec...

Synthesis and Functionalization of Gold Nanoparticles by Using of Poly Functional Amino Acids

Synthesis and characterization of two functionalized gold nanoparticles by using of two poly functional amino acids (L-Arginine and L-Aspartic acid) are reported. The gold nanoparticles were reduced by sodium citrate and functionalized with L-Arginine at the pH of 7 and 11 and L-Aspartic acid at the pH of 7. Transmission electron microscopy, UV-Vis spectroscopy, dynamic light scattering, zeta p...