Regulation of Release of Hepatocyte Growth Factor From Human Promyelocytic Leukemia Cells, HL-60, by 1,25-Dihydroxyvitamin D,, 12-0-Tetradecanoylphorbol 13-Acetate, and Dibutyryl Cyclic Adenosine Monophosphate

Hepatocyte growth factor (HGF) secreted from human promyelocytic leukemia cell line, HL-60, is indistinguishable from HGF in human plasma and its release is significantly stimulated by 1 2-0-tetradecanoylphorbol-I 3-acetate (TPA), a differentiation-inducer of HL-60 cells into monocytes/macrophages (Nishino T et al: Biochem Biophys Res Commun 181 :323, 1991). TPA stimulated HGF release from the cells through an activation of C-kinase, but not through a formation of reactive oxygen species. Furthermore, dibutyryl cAMP (dbcAMP), an activator of A-kinase and granulocyte-inducer, also stimulated HGF release. 1 ,SB-Dihydroxyvitamin D3, another monocyte/ macrophage-inducer, abated either TPAor dbcAMP-stimulated synthesis and release of HGF in a dose-dependent manner probably via its nuclear receptor as reflected by