Absence of polyadenylic acid from reovirus messenger ribonucleic acid.

نویسندگان

  • C M Stoltzfus
  • A J Shatkin
  • A K Banerjee
چکیده

Reovirus single-stranded RNA isolated from mouse L cells pulse-labeled with radioactive RNA precursors for various intervals at different times after infection does not contain detectable poly(A) sequences. All three size classes of viral mRNA, like reovirus double-stranded genome RNA, have cytosine at their 3’ termini. The results indicate that the presence of poly(A) sequences in reovirus single-stranded RNA is not required for translation or conversion to doublestranded viral RNA.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Structure and function of the reovirus genome.

INTRODUCTION ................ ................................ 483 DISCOVERY OF REOVIRUSES .............................................. 483 STRUCTURE OF THE REOVIRUS PARTICLE .................. ............. 484 REOVIRUS GENOME ................................................ 484 PROTEINS OF REOVIRUS ................................................ 486 INFORMATION CONTENT OF THE 10 REOVIRUS G...

متن کامل

Improved methods for purification and assay of eukaryotic messenger ribonucleic acids and ribosomes. Quantitative analysis of their interaction in a fractionated reticulocyte cell-free system.

The polyadenylic acid-containing messenger ribonucleic acids of eukaryotic cells are rapidly isolated and deproteinized in a simple and gentle one-step procedure. The polyribosome fraction, dissolved in 0.5 M NaCl/0.5 percent sodium dodecyl sulfate, is passed through an oligo(dT)-cellulose column which is then washed with the solvent until proteins and contaminating ribonucleic acids are fully ...

متن کامل

Protein synthesis in a cell-free extract from Staphylococcus aureus.

Cell-free Staphylococcus aureus extracts have been prepared which actively incorporate amino acids into protein. The requirements for amino acid incorporation of this preparation were strongly suggestive of de novo protein synthesis, since it showed an absolute requirement for ribosomes, 105,000 x g supernatant fluid, energy source, and magnesium ion. The stability of these extracts was greatly...

متن کامل

Absence of adenine-rich ribonucleic acid from purified infectious reovirus 3.

The basic properties of released and cell-associated reovirus are the same. Both contained as their total nucleic acid complement only double-stranded ribonucleic acid (RNA) with an adenine content of 28%. Preparations of purified cell-associated virus, but not released virus, contained adenine-rich RNA which could be separated from the virus with little or no loss of infectivity. These adenine...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • The Journal of biological chemistry

دوره 248 23  شماره 

صفحات  -

تاریخ انتشار 1973