Animal-cell damage in sparged bioreactors.

Characterization of the localized hydrodynamic shear forces and dissolved oxygen distribution in sparged bioreactors.

Detailed, high-resolution numerical simulations of the bubbly flows, used for oxygen delivery and mixing in mammalian cell suspensions, have been performed. The hydrodynamics, shear and normal forces, mass transfer and mass transport from and around individual bubbles and bubble clusters were resolved for different operating conditions, that is, Weber, Morton, and Schmidt numbers. Suspended ani...

Pii: 0032-9592(93)85012-5

The scale-up of stirred tank bioreactors from 0.02 m3 to a 03 m-’ commercial plant is discussed for hybridoma suspension cultures. Schemes for dissolved oxygen control with sparged air in serum containing media are described, as well as mechanical breakage offoam in small and large bioreactors. Porous metal spargers (180-200 x lo6 m) were found to produce foams which were hard to control. Aerat...

Evaluation of a novel Wave Bioreactor cellbag for aerobic yeast cultivation.

The Wave Bioreactor is widely used in cell culture due to the benefits of disposable technology and ease of use. A novel cellbag was developed featuring a frit sparger to increase the system's oxygen transfer. The purpose of this work was to evaluate the sparged cellbag for yeast cultivation. Oxygen mass transfer studies were conducted in simulated culture medium and the sparged system's maximu...

Dynamic Simulation and Control of Microbial Cell Population in Continuous Bioreactors

Microfluidic assessment of mechanical cell damage by extensional stress.

Mammalian cells have been widely used in bioreactors to produce biological products such as pharmaceutical materials. The productivity of such bioreactors is vastly affected by flow-induced cell damage in complicated flow environments, such as agitation-driven turbulence and oxygen bubble bursting at the interface between the culturing medium and air. However, there is no systematic approach to...