Thiolated uridine substrates and templates improve the rate and fidelity of ribozyme-catalyzed RNA copying.
نویسندگان
چکیده
Ribozyme-catalyzed RNA polymerization is inefficient and error prone. Here we demonstrate that two alternative bases, 2-thio-uridine (s(2)U) and 2-thio-ribo-thymidine (s(2)T), improve the rate and fidelity of ribozyme catalyzed nucleotide addition as NTP substrates and as template bases. We also demonstrate the functionality of s(2)U and s(2)T-containing ribozymes.
منابع مشابه
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عنوان ژورنال:
- Chemical communications
دوره 52 39 شماره
صفحات -
تاریخ انتشار 2016