Sch 31828, a novel antibiotic from a Microbispora sp.: taxonomy, fermentation, isolation and biological properties.

lated from a mixture of soils plated on yeast starch agar (yeast extract 0.5 %, soluble starch 1.0%, Difco agar 1.5%, pH 7.0) containing 25 jug/ml everninomicin. On the descriptive media of Shirling and Gottlieb5), and Waksman6), after 21 days at 30°C, the culture forms beige to tan vegetative mycelia pigments, gray-white to pink aerial mycelia and a faint yellow-brown diffusible pigment. Upon incubation for an additional 7 to 14 days, the aerial mycelia turn gray-green. Microscopically the substrate mycelia are long, irregularly branched, do not fragment into short elements, or form spores. The aerial hyphae branch monopodially. Elliptical spores are born in longitudinal pairs closely arranged along the aerial hyphae. The