Next-generation Microfilter: Large Scale, Conti- Nuous Mammalian Cell Retention for Perfusion Bio- Reactors
نویسندگان
چکیده
In this study, we report on the development of the first membrane-less, clog-free microfiltration platform for ultra-high throughput (up to 1000 mL/min) cell separation, using a massively-multiplexed array of inertial microfluidic cell sorting channels. Our developed system consists of multiple layer (10~20) of PDMS sheets with embossed microchannels (i.e., ~ 200 individual spirals) bonded together for continuous size-based cell sorting from a large volume of biological fluid. Subsequent perfusion culture experiments using the cell retention system show the potential to significantly enhance overall efficiency of perfusion cell culture.
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تاریخ انتشار 2014