Acquired tolerance to oxidative stress in Bifidobacterium longum 105-A via expression of a catalase gene.
نویسندگان
چکیده
For improvement of tolerance to oxidative stress in Bifidobacterium longum 105-A, we introduced the Bacillus subtilis catalase gene (katE) into it. The transformant showed catalase activity (39 U/mg crude protein) in the intracellular fraction, which increased survival by ∼100-fold after a 1-h exposure to 4.4 mM H(2)O(2), decreased de novo H(2)O(2) accumulation, and increased survival in aerated cultures by 10(5)-fold at 24 h. The protection level was better than that conferred by exogenously added catalase.
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عنوان ژورنال:
- Applied and environmental microbiology
دوره 78 8 شماره
صفحات -
تاریخ انتشار 2012