Layer by layer assembly of biotinylated protein networks for signal amplification.
نویسندگان
چکیده
Described herein is a unique and inexpensive method that outperforms commercial methods that amplify the streptavidin-biotin recognition event. Amplification induced by streptavidin and biotinylated protein causes the formation of a large detectable polymer. This approach enjoys a 100-fold decrease in detection limit in comparison with the commercial methods.
منابع مشابه
Second Harmonic Generation Diagnostic of Layer by Layer Deposition from Disperse Red 1–Functionalized Maleic Anhydride Copolymer
Layer-by-layer (LBL) electrostatic assembly of poly-electrolytes is proving to be an increasingly rich and versatile technique for the formation of multilayered thin films with a wide range of electrical, magnetic, and optical properties. In the present work we synthesized a new nonlinear optical (NLO) maleic acid copolymer containing Disperse Red 1 moieties, built-up multilayer assemblies by a...
متن کاملProtein and Lactose Separation by Modified Ultrafiltration Membrane using Layer by Layer Technique
Layer-by-Layer (LbL) is a method which can be used for nanoscale coating and surface functionalization of a material. LbL technique mainly uses the electrostatic attracting between charged materials (polyelectrolytes, nanoparticles, etc.) and an oppositely charged surface. In this study, protein separation (BSA) from lactose solution was carried out using the LbL self-assembly method, which was...
متن کاملDamage detection and structural health monitoring of ST-37 plate using smart materials and signal processing by artificial neural networks
Structural health monitoring (SHM) systems operate online and test different materials using ultrasonic guided waves and piezoelectric smart materials. These systems are permanently installed on the structures and display information on the monitor screen. The user informs the engineers of the existing damage after observing signal loss which appears after damage is caused. In this paper health...
متن کاملThe Application of Multi-Layer Artificial Neural Networks in Speckle Reduction (Methodology)
Optical Coherence Tomography (OCT) uses the spatial and temporal coherence properties of optical waves backscattered from a tissue sample to form an image. An inherent characteristic of coherent imaging is the presence of speckle noise. In this study we use a new ensemble framework which is a combination of several Multi-Layer Perceptron (MLP) neural networks to denoise OCT images. The noise is...
متن کاملDetection of immunoglobulin light chain mRNA by in situ hybridisation using biotinylated tyramine signal amplification.
A highly sensitive method for the light microscopic in situ hybridisation of immunoglobulin light chain mRNA in formalin fixed, paraffin wax embedded sections is reported. This method is based on signal amplification using horseradish peroxidase catalysed deposition of biotinylated tyramine at the sites of hybridisation. kappa and lambda light chain immunoglobulin mRNA in situ hybridisation was...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Chemical communications
دوره 49 24 شماره
صفحات -
تاریخ انتشار 2013