Selective blotting of restriction DNA fragments on nitrocellulose membranes at low salt concentrations.
نویسندگان
چکیده
The pattern of restriction DNA fragments transferred from agarose gel to nitrocellulose membranes (Southern's technique) can be affected by the salt concentration of the transfer solvent.
منابع مشابه
Southern Blotting and Related DNA Detection Techniques
Southern blotting is one of the central techniques in molecular biology. First devised by E.M. Southern (1975), Southern blotting results in transfer of DNA molecules, usually restriction fragments, from an electrophoresis gel to a nitrocellulose or nylon sheet (referred to as a ‘membrane’), in such a way that the DNA banding pattern present in the gel is reproduced on the membrane. During tran...
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عنوان ژورنال:
- Nucleic acids research
دوره 8 11 شماره
صفحات -
تاریخ انتشار 1980