Studies on the Secondary Structure of Intraphage T-7 DNA
ثبت نشده
چکیده
Secondary structure of intraphage DNA of coliphage T-7 was studied by spectrophotometry and electron microscopy. T-7 DNA in vivo showed 1% hyperchromicity, on the average, compared to DNA released by heating the phage 30 min at 55 — 60 °C. The intraphage DNA when melted by heating the phage for 13 min at 95° and then rapidly chilled, was found to be defficient in hyperchromic rise by about 10% compared to isolated T-7-DNA treated under similar conditions. Electron micrographs of T-7-DNA released by osmotic shock revealed that the incompletely released molecules showing a pattern possessed denatured regions in the form of single-stranded loops. Phenol extracted T-7-DNA molecules as well as T-7 genomes completely released by osmotic shock did not show any pattern and possessed no denatured zones. These results helped to infer that intraphage T-7-DNA is partially denatured.
منابع مشابه
Phylogenetic Analysis of Beta-Glucanase Producing Actinomycetes Strain TBG-CH22 - A Comparison of Conventional and Molecular Morphometric Approach
Actinomycetes are inexhaustible producers of commercially valuable metabolites, are continually screened for beneficial compounds. The taxonomic and phylogenetic study of novel actinomycetes strains are mostly based on conventional methods and primary DNA structure of 16s rRNA. Although 16s rRNA sequence is well accepted in phylogeny studies, its secondary structures have not been widely used. ...
متن کاملPhylogenetic Analysis of Beta-Glucanase Producing Actinomycetes Strain TBG-CH22 - A Comparison of Conventional and Molecular Morphometric Approach
Actinomycetes are inexhaustible producers of commercially valuable metabolites, are continually screened for beneficial compounds. The taxonomic and phylogenetic study of novel actinomycetes strains are mostly based on conventional methods and primary DNA structure of 16s rRNA. Although 16s rRNA sequence is well accepted in phylogeny studies, its secondary structures have not been widely used. ...
متن کاملEffect of LIGHT Adjuvant on Kinetics of T-Cell Responses Induced by HSV-1 DNA Immunization
Background: Studies on efficacy of various vaccines that prevent or reduce the primary and recurrent HSV-1 infection have demonstrated the importance of cellular immunity for protection against the infection. We previously used DNA vaccination to induce cellular immunity against HSV-1 infection in mice. Objective: The aim of our study was to evaluate the effect of LIGHT, a member of TNF super f...
متن کاملConstruction of pIRES2 vector encoding truncated NS3 of HCV and IL-18 for DNA vaccine studies
Introduction: Long term complications of hepatitis C virus (HCV) infection include fibrosis, cirrhosis, and hepatocellular carcinoma and although the disease is treatable with the newly introduced direct acting antivirals, but factors such as drug resistance, viral genotype and adverse effects can limit the effectiveness of therapy. Therefore, development of effective, safe and affordable proph...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2015