Immunomodulatory activity of acidic polysaccharides isolated from Tanacetum vulgare L.
Tanacetum vulgare L. (Tansy) has been extensively used in folk medicine for treatment of a variety of medical disorders. In the present study, we isolated and purified four acidic polysaccharide fractions (designated T-I to T-IV) from Tansy florets by the sequential use of hot-water extraction, ethanol precipitation, ultra-filtration, anion-exchange, and size-exclusion chromatography. The average M(r) of fractions T-I through T-IV was estimated to be 326, 151, 64 and 9 kDa, respectively, as determined by high performance size-exclusion chromatography analysis. Sugar composition analysis revealed that Tansy polysaccharides consisted primarily of galacturonic acid, galactose, arabinose, and rhamnose. Fractions T-II through T-IV contained an arabinogalactan type II structure, as determined by reaction with Yariv reagent. High M(r) fractions T-I and T-II exhibited potent macrophage/monocyte-activating activity, enhancing production of reactive oxygen species (ROS), nitric oxide (NO), and tumor necrosis factor alpha (TNF-alpha) by J774.A1 murine macrophages, and activating nuclear factor kappaB (NF-kappaB) in THP-1 human monocytes. In addition, Tansy polysaccharides stimulated human neutrophil function by greatly enhancing neutrophil myeloperoxidase (MPO) release. Furthermore, the low M(r) fraction T-IV had potent complement-fixing activity, which may also contribute to the anti-inflammatory and would-healing properties of Tansy extracts. Taken together, our results provide a molecular basis to explain at least part of the beneficial therapeutic effects of Tansy extracts, and support the concept of using Tansy polysaccharides as an immunotherapeutic adjuvant.
The methanolic extract of aerial parts of Tanacetum vulgare L. (Asteraceae) and its fractions were investigated for antioxidant activity. The crude extract displayed DPPH radical scavenging effects with an EC50 value of 37 +/- 1.2 microg/mL (n=3). Activity-guided fractionations of the crude extract resulted in the isolation of three antioxidant compounds; 3,5-O-dicaffeoylquinic acid (3,5-DCQA),...متن کامل
A phytochemical analysis of the dichloromethane extract from the flowers of a subspecies of Tanacetum vulgare growing in Sicily was carried out. Five known sesquiterpene lactones with the eudesmane skeleton have been isolated and the cytotoxic activity of these compounds was tested in vitro on A549 (human lung carcinoma epithelial-like) and V79379A (Chinese hamster lung fibroblast-like) cells u...متن کامل
The stereospecificity of the oxidation of trans-hex-2-en-l-01 and ethanol was determined for alcohol oxidases from Tanacetum vulgare leaves and from the yeast Candida boidinii. It was found that both enzymes remove the pro-1R hydrogen. The enzyme from Candida boidinii also removes the pro-1S hydrogen but t!o a much lesser degree.متن کامل
Antileishmanial activity of parthenolide, a sesquiterpene lactone isolated from Tanacetum parthenium.
The in vitro activity of parthenolide against Leishmania amazonensis was investigated. Parthenolide is a sesquiterpene lactone purified from the hydroalcoholic extract of aerial parts of Tanacetum parthenium. This isolated compound was identified through spectral analyses by UV, infrared, (1)H and (13)C nuclear magnetic resonance imaging, DEPT (distortionless enhancement by polarization transfe...متن کامل
In vitro cytotoxicity of Tanacetum vulgare mediated silver nanoparticles against breast cancer (MCF-7) cell lines
Purpose: The present investigation describes the biosynthesis of Silver nanoparticles (AgNPs) using Tanacetum vulgare leaf extract by an environmental-friendly method. Also, the in-vitro cytotoxicity of synthesized AgNPs towards the human breast cancer (MCF-7) cell lines. Methods: Ag NPs were prepared from AgNO3 using Tanacetum vulgare leaf extract polyphenols as reducing and capping agent. Fur...متن کامل