Electroimmunodiffusion — a Powerful Tool for Quantitative Determinations of Both Soluble and Membrane Bound Chloroplast ATPase, CF j
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چکیده
Electroimmunodiffusion, Chloroplast, C F r Content 1. Electroimmunodiffusion can be used for routine specific quantitative microdeterminations of photosynthetic ATP-synthase. The method has been worked out for samples of both the dissociated (C F ,) and the membrane bound enzyme (C F 0C F ,) . 2. Only a monospecific antiserum against C F , is required for relative determinations of C F] in any fraction. If beyond this an absolute determination is intended of either the separated or the membrane bound ATPase, in addition a pure C F , is needed for calibration. 3. A varying CF,/chlorophyll ratio between 1/700 and 1/1200 has been found for thylakoids of spinach plants grown under various light intensities. 4. Since the amount of C F , was the limiting factor o f photophosphorylation capacity in the electron transport system used (PM S), from simultaneous measurement of C F , content and photophosphorylation capacity a turn over time of the ATPsynthase of 4 m s /A T P /C F 0C F , could be recalculated.
منابع مشابه
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تاریخ انتشار 2013