A Streamlined Technique to Examine Cell Monolayers by means of Correlative Light and Transmission Electron Microscopy
نویسندگان
چکیده
Correlative light/electron microscopy (CLEM) combines the power of light microscopy to search large areas for fluorescently-tagged live cells/regions of interest and transmission electron microscopy (TEM) to image the same specimen at high magnification for ultrastructural characterization. CLEM methods are essential to observe dynamic processes such as organelle biogenesis and intracellular trafficking and then investigate the same cell regions with the resolution of the TEM. Although previously described techniques can be technically demanding, a simplified protocol would facilitate CLEM in nonspecialized laboratories.
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Light and electron microscope of grass carp (Ctenopharyngodon idella) organs following exposure to various sublethal concentrations of diazinon
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تاریخ انتشار 2014