DNA sequence fine-structure analysis of ilvG (IlvG+) mutations of Escherichia coli K-12.
نویسندگان
چکیده
Six ilvG (IlvG+) mutations of Escherichia coli K-12 were transferred to recombinant plasmids, and the DNA sequence of each mutation was determined. This analysis confirmed that expression of the ilvG gene product (acetohydroxy acid synthase II) requires the deletion of a single base pair or the addition of two base pairs within ilvG to displace a frameshift site present in wild-type E. coli K-12. This system should be useful in the analysis of potential frameshift mutagens.
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عنوان ژورنال:
- Journal of bacteriology
دوره 149 1 شماره
صفحات -
تاریخ انتشار 1982