Control of gluconate utilization in Sinorhizobium meliloti.
نویسندگان
چکیده
The Sinorhizobium meliloti megaplasmid pSymA has previously been implicated in gluconate utilization. We report a locus on pSymA encoding a putative tripartite ATP-independent periplasmic (TRAP) transporter that is required for gluconate utilization. The expression of this locus is negatively regulated by a GntR family regulator encoded adjacent to the transporter operon.
منابع مشابه
تأثیر شوری بر برخی شاخص‌های رشد و پروتئین کل یونجه تلقیح شده با جدایه‌های باکتری Sinorhizobium meliloti در شرایط گلخانه
This greenhouse experiment was carried out to evaluate the effects of salinity and bacterial inoculation on some growth indices and total protein content of alfalfa (Medicago sativa) using a factorial completely randomized design with three replications. The effect of three salinity levels (0, 6 and 12 dS/m) induced by a mixture of NaCl, CaCl2 and MgCl2 salts on growth indices and protein conte...
متن کاملAn AraC-like transcriptional activator is required for induction of genes needed for alpha-galactoside utilization in Sinorhizobium meliloti.
The nodulating bacterium Sinorhizobium meliloti can utilize alpha-galactosides like melibiose and raffinose as sole sources of carbon and energy. We show that this utilization requires an AraC-like transcriptional activator, AgpT. When agpT was inactivated, Rhizobium meliloti could not utilize alpha-galactosides or induce genes required for transport and catabolism of these sugars. The agpT gen...
متن کاملIdentification of an acetoacetyl coenzyme A synthetase-dependent pathway for utilization of L-(+)-3-hydroxybutyrate in Sinorhizobium meliloti.
D-(-)-3-Hydroxybutyrate (DHB), the immediate depolymerization product of the intracellular carbon store poly-3-hydroxybutyrate (PHB), is oxidized by the enzyme 3-hydroxybutyrate dehydrogenase to acetoacetate (AA) in the PHB degradation pathway. Externally supplied DHB can serve as a sole source of carbon and energy to support the growth of Sinorhizobium meliloti. In contrast, wild-type S. melil...
متن کاملShmR is essential for utilization of heme as a nutritional iron source in Sinorhizobium meliloti.
The bacterium Sinorhizobium meliloti is able to use heme as a nutritional iron source. Here, we show that the iron-regulated shmR gene encodes an outer membrane protein required for growth on heme. Furthermore, an shmR mutant is resistant to the toxic heme analog gallium protoporphyrin. Thus, the receptor protein of the heme transport system has been identified in S. meliloti.
متن کاملMetabolic modelling reveals the specialization of secondary replicons for niche adaptation in Sinorhizobium meliloti
The genome of about 10% of bacterial species is divided among two or more large chromosome-sized replicons. The contribution of each replicon to the microbial life cycle (for example, environmental adaptations and/or niche switching) remains unclear. Here we report a genome-scale metabolic model of the legume symbiont Sinorhizobium meliloti that is integrated with carbon utilization data for 1,...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Journal of bacteriology
دوره 191 4 شماره
صفحات -
تاریخ انتشار 2009