Determination of P (F11) and F1 fimbriae of Escherichia coli isolated from avian cellulitis
نویسندگان
چکیده
Avian cellulitis has been induced by virulent strains of E. coli. The purpose of this study was to determine P (F11) and F1 fimbriae of 90 E. coli isolates of avian cellulitis. Isolates were subjected to six consecutive passages on solid and static broth for expression of fimbriae. Five (5.5%) isolates from O1 serogroup, showed a mannose-resistant haemagglutination MRHA pattern when grown on solid medium. In the SDS-PAGE, crude fimbrial extracts of MRHA strain showed a major fimbrial subunit of 18 kDa. This band was also reacted with anti F11 serum on immunoblotting. Sixty-nine (76.6%) E. coli isolates from different serogroups showed mannose-sensitive hemagglutinating (MSHA) pattern when grown on static broth medium. In immunoblotting test, crude fimbrial extracts of MSHA isolates demonstrated a single band with 17 to 17.5 kDa apparent molecular weight as revealed by absorbed anti-F1A serum. It would appear that avian cellulitis E. coli isolates have F1 and P fimbriae similar to those of colisepticemic E. coli isolates.
منابع مشابه
Purification and characterization of P fimbriae from an Escherichia coli strain isolated from a septicemic turkey.
A pap+ Escherichia coli isolate from a turkey with colisepticemia expressed P fimbriae with a major subunit of an apparent molecular mass of 18 kDa which reacted with anti-F11 serum. This fimbriae was purified and polyclonal antiserum was produced in rabbits. The N-terminal amino acid sequence of the major fimbrial subunit of the avian P fimbriae was identical to that of F11. On immunoblotting,...
متن کاملECOR phylotyping and determination of virulence genes in Escherichia coli isolates from pathological conditions of broiler chickens in poultry slaughter-houses of southeast of Iran
Avian pathogenic Escherichia coli (APEC) are responsible for wide ranges of extra-intestinal diseases in poultry including colibacillosis, cellulitis, coligranuloma and yolk sac infection. Numbers of virulence are considered important in the pathogenicity of these diseases. The aims of the present study were phylogenetic typing and virulence genes detection in Escherichia coli </em...
متن کاملFrequency of selected virulence-associated genes in intestinal and extra-intestinal Escherichia coli isolates from chicken
Background: Although Escherichia coli (E. coli)is a part of intestinal normal microflora of warm-blooded animals, includingpoultry, outbreaks occur in poultry raised below standard sanitation and duringthe course of respiratory or immunosuppressive diseases. Avian pathogenic E.coli (APEC) harbors several genes associated with virulence andpathogenicity. APEC strains are responsible for some dis...
متن کاملMolecular Study of Phase Variation of Type 1 Fimbriae in Uropathogenic Escherichia coli O44 Serotypes during Touching with Solid Surfaces
Background & Aims: Type 1 fimbriae is the most common adhesion factor in urine tract infection. In this Study, presence of virulence genes in isolated strains of uropathogenic E.Coli, O serotyping and molecular detection of phase variation of type 1 fimbriae were assessed during solid surfaces exposure. Methods: Isolated E.coli from urine samples of patients were serotyped by using serologic me...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2003