Comparison of the isoelectric focusing patterns of darbepoetin alfa, recombinant human erythropoietin, and endogenous erythropoietin from human urine.

نویسندگان

  • Don H Catlin
  • Andreas Breidbach
  • Steve Elliott
  • John Glaspy
چکیده

rejection by elevated circulating levels of serum intestinal fatty acid binding protein. Surgery 1993;114:206–10. 12. Ascher N, Stock PG, Baumgarder GL, Payne WD, Najarian JS. Infection and rejection of primary hepatic transplant in 93 consecutive patients treated with triple immunosuppressive therapy. Surg Gynecol Obstet 1988;167: 474–84. 13. Hughes VF, Trull AK, Joshi O, Alexander GJM. Monitoring eosinophil activation and liver function following liver transplantation. Transplantation 1998; 65:1334–9. 14. Wodzig KW, Kragten JA, Hermens WT, Glatz JF, van Dieijen-Visser MP. Estimation of myocardial infarct size from plasma myoglobin or fatty acid-binding protein: influence of renal function. Eur J Clin Chem Biochem 1997;35:191–8. 15. Rees GW, Trull AK, Doyle S. Evaluation of an enzyme-immunometric assay for serum -glutathione S-transferase. Ann Clin Biochem 1995;32:575–83. 16. Schreiber A, Feldbrugge R, Key G, Glatz JF, Spener F. An immunosensor based on disposable electrodes for rapid estimation of fatty acid-binding protein, an early marker of myocardial infarction. Biosens Bioelectron 1997;12:1131–7. 17. Key G, Schreiber A, Feldbrugge R, McNeil CJ, Jorgenson P, Pelsers MM, et al. Multicenter evaluation of an amperometric immunosensor for plasma fatty acid-binding protein: an early marker for acute myocardial infarction. Clin Biochem 1999;32:229–31. 18. Robers M, van der Hulst FF, Fischer M, Roos W, Salud CE, Eisenwiener HG, et al. Development of a rapid microparticle-enhanced turbidimetric immunoassay for fatty acid-binding protein in plasma, an early marker of acute myocardial infarction. Clin Chem 1998;44:1564–7.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Detection of recombinant human erythropoietin in urine by isoelectric focusing.

BACKGROUND Doping with erythropoietic proteins such as recombinant human erythropoietin (rHuEPO) and darbepoetin alfa is a serious issue in sport. There is little information on the time course of detection of rHuEPO in urine and on methods to evaluate electrophoresis-based data. METHODS We used a recently described isoelectric focusing method for detecting rHuEPO and endogenous EPO in urine ...

متن کامل

Assessing the instability of the isoelectric focusing patterns of erythropoietin in urine.

IEF can be used to differentiate human urinary erythropoietin (uEPO), recombinant human erythropoietin or epoetin (rEPO) and darbepoetin (novel erythropoiesis stimulating protein (NESP)). This is the basis of the method currently used to detect misuse of rEPO and NESP by elite athletes. Recently, an unknown activity has been attributed to some urine samples (denominated 'unstable' urine by the ...

متن کامل

Brief report False-positive detection of recombinant human erythropoietin in urine following strenuous physical exercise

Erythropoietin (Epo) is a glycoprotein hormone that promotes the production of red blood cells. Recombinant human Epo (rhEpo) is illicitly used to improve performance in endurance sports. Doping in sports is discouraged by the screening of athletes for rhEPO in urine. The adopted test is based on a combination of isoelectric focusing and double immunoblotting, and distinguishes between endogeno...

متن کامل

False-positive detection of recombinant human erythropoietin in urine following strenuous physical exercise.

Erythropoietin (Epo) is a glycoprotein hormone that promotes the production of red blood cells. Recombinant human Epo (rhEpo) is illicitly used to improve performance in endurance sports. Doping in sports is discouraged by the screening of athletes for rhEPO in urine. The adopted test is based on a combination of isoelectric focusing and double immunoblotting, and distinguishes between endogeno...

متن کامل

Detection of recombinant epoetin and darbepoetin alpha after subcutaneous administration in the horse.

A direct detection method for anti-doping control of recombinant human erythropoietin (rHuEPO) abuse in racehorses is proposed. This method involves screening of plasma (or serum) by an enzyme-linked immunosorbent assay specific for human EPO and confirmation in urine samples by characterization of the urinary EPO isoelectric profile. This method was tested on horses that were administered epoe...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Clinical chemistry

دوره 48 11  شماره 

صفحات  -

تاریخ انتشار 2002