Reactivity of vertebrate - directed phospho - eEF 2 antibody against the orthologue phospho - EEF - 2 Caenorhabditis elegans

Eukaryotic protein translation is divided into three mains stages: initiation, elongation and termination. Regulation of this process occurs at the initiation and elongation step. eEF2 kinase phosphorylates eEF2 factor, blocking its ribosome interaction and thus translation elongation. This kinase activity can be detected by measuring eEF2 phosphorylation status. Here I show that vertebrate-specific antibody against phospho-eEF2 has excellent reactivity against orthologue protein phospho-EEF-2. C. elegans Viviane Alves ( ) Corresponding author: [email protected] Competing interests: The author declares no competing interests. Alves V. How to cite this article: Reactivity of vertebrate-directed phospho-eEF2 antibody against the Caenorhabditis elegans 2015, :902 (doi: ) orthologue phospho-EEF-2 [version 1; referees: 2 approved] F1000Research 4 10.12688/f1000research.7127.1 © 2015 Alves V. This is an open access article distributed under the terms of the , which permits Copyright: Creative Commons Attribution Licence unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. This work was supported by CNPq – Centro Nacional de Desenvolvimento Cientifico e Tecnologico, Capes – Coordenaçao de Grant information: Aperfeiçoamento de Pessoal de nível Superior, and PRPq-UFMG – Pro-reitoria de Pesquisa da Universidade Federal de Minas Gerais. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. 25 Sep 2015, :902 (doi: ) First published: 4 10.12688/f1000research.7127.1 Referee Status: