Pneumococci of More Than One Capsular Type

نویسندگان

  • LARRY S. McDANIEL
  • JEANNE S. SHEFFIELD
  • PAMELA DELUCCHI
چکیده

Monoclonal antibodies against pneumococcal surface protein A (PspA) have been shown to protect mice from fatal pneumococcal infection. PspA is highly variable serologically, raising the possibility that PspA from one strain might not be able to elicit protective responses against strains which possess serologically different PspA. We have prepared a Agtll library of pneumococcal genomic DNA and identified a clone expressing PspA. The recombinant PspA in this phage lysate elicited protection against pnehmococcal infections with three strains of two different capsular serotypes. This finding demonstrated that PspA could elicit a protective response in the absence of other pneumococcal antigens. The observed protection was probably antibody mediated because it could be passively transferred with immune sera. Lambda lysates producing pneumococcal proteins other than PspA failed to elicit protection against fatal pneumococcal infection.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Control by Factors Distinct from the S Transforming Principle of the Amount of Capsular Polysaccharide Produced by Type Iii Pneumococci

Pneumococci can possess the normal S transforming principle and yet produce amounts of capsular polysaccharide much less than would be expected if the quantity formed depended only on the nature of the S transforming principle.

متن کامل

The Effect of the Bacteriolytic Enzyme of Pneumococcus upon the Antigenicity of Encapsulated Pneumococci

1. Mice immunized with heat-killed cells of virulent pneumococci (Type I) which have been treated with active preparations of the bacteriolytic enzyme, develop a certain degree of type specific resistance to subsequent infection. This active immunity, however, appears to be due to the small amount of free acetyl polysaccharide present in the suspension of digested bacteria, and is always of a l...

متن کامل

Illustration of pneumococcal polysaccharide capsule during adherence and invasion of epithelial cells.

The capsular polysaccharide of Streptococcus pneumoniae represents an important virulence factor and protects against phagocytosis. In this study the amount of capsular polysaccharide present on the bacterial surface during the infection process was illustrated by electron microscopic studies. After infection of A549 cells (type II pneumocytes) and HEp-2 epithelial cells a modified fixation met...

متن کامل

Interaction of pneumococcal antigens with complement in rats.

Complement activation with pneumococcal antigens was studied both in vitro and after injection of the antigens into rats. Whole pneumococci of various serotypes activated C3-C9 in rat serum treated with ethyleneglycol-bis (beta-aminoethyl ether)-N,N'-tetraacetic acid, although serotypes differed greatly in the extent of activation. Some purified pneumococcal capsular polysaccharides also activa...

متن کامل

The Transformation in Vitro of R Pneumococci into S Forms of Different Specific Types by the Use of Filtered Pneumococcus Extracts

1. Avirulent R pneumococci derived from S forms of a specific type may be changed by growth in broth containing anti-R serum and a heated, filtered extract of S pneumococci of a different type, into virulent S organisms identical in type with the bacteria extracted. This has been accomplished in the case of R strains derived from Type II pneumococci, employing extracts prepared from Type III an...

متن کامل

Stability of serotypes during nasopharyngeal carriage of Streptococcus pneumoniae.

Serotype changes among natural isolates of Streptococcus pneumoniae are well documented and occur by recombinational exchanges at the capsular biosynthetic locus. However, the frequency with which this phenomenon occurs within the nasopharynx of children is not clear and is likely to be highest in the nasopharynx of children, who have high rates of pneumococcal carriage. A birth cohort of 100 i...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2003