Spatiotemporal Monitoring of Pseudomonas Effectors via Type III Secretion Using Split Fluorescent Protein Fragments

Pathogenic gram-negative bacteria cause serious diseases in animals and plants. These bacterial pathogens use the type III secretion system (T3SS) to deliver effector proteins into host cells; these effectors then localize to different subcellular compartments to attenuate immune responses by altering biological processes of the host cells. The fluorescent protein (FP)-based approach to monitor effectors secreted from bacteria into the host cells is not possible because the folded FP prevents effector delivery through the T3SS. Therefore, we optimized an improved variant of self-assembling split superfolder green fluorescent protein (sfGFP) system to investigate the spatiotemporal dynamics of effectors delivered through bacterial T3SS into plant cells. In this system, effectors are fused to 11th β-strand of super-folder GFP (sfGFP11) and when delivered into plant cells expressing sfGFP1-10 β -strand (sfGFP1-10), the two proteins reconstitute GFP fluorescence. We generated a number of Arabidopsis thaliana transgenic lines expressing sfGFP1-10 targeted to various subcellular compartments to facilitate localization of GFP11tagged effectors delivered from bacteria. We demonstrate the efficacy of this system using Pseudomonas effectors AvrB and AvrRps4 in Nicotiana benthamiana and transgenic Arabidopsis plants. The versatile split sfGFP system described here will facilitate a better understanding of bacterial invasion strategies used to evade plant immune responses. Plant Cell Advance Publication. Published on June 14, 2017, doi:10.1105/tpc.17.00047 ©2017 American Society of Plant Biologists. All Rights Reserved