Glass Micropallets for Adherent Cell Analysis and Recovery

This paper presents a novel microtechnology, glass micropallets, for the recovery of single adherent cells from mixed cellular populations. This work significantly advances the base technology, the micropallet array, which has been demonstrated for the analysis of adherent cell populations and isolation and selection of individual cells. We report a complete departure from the photolithographic fabrication methods used previously and present a micropallet array made of glass instead of polymeric materials, which improves the technology in several aspects. We demonstrate the glass micropallet array’s improved optical properties and its ability to selectively recover adherent cells from large populations.