mutants in a Canton-S genetic background did not survive as long on agar as flies fed with 1% ethanol. Consistent with the data for wild-type Canton-S, the ethanol fed flies

single male fly was housed in inner vial, with water in outer vial, to keep a high humidity inside. Two kinds of liquid food were provided to the fly through two capillaries separately. One contains 5% sucrose and 5% yeast extract, which is represented by green color. The other contains 5% sucrose, 5% yeast extract and ethanol in a certain concentration, which is represented by red color. The two capillaries were replaced every 24 h with their locations were exchanged.