Shear-wave elasticity measurements of three-dimensional cell cultures for mechanobiology
نویسندگان
چکیده
Studying mechanobiology in three-dimensional (3D) cell cultures better recapitulates cell behaviors in response to various types of mechanical stimuli in vivo Stiffening of the extracellular matrix resulting from cell remodeling potentiates many pathological conditions, including advanced cancers. However, an effective tool for measuring the spatiotemporal changes in elastic properties of such 3D cell cultures without directly contacting the samples has not been reported previously. We describe an ultrasonic shear-wave-based platform for quantitatively evaluating the spatiotemporal dynamics of the elasticity of a matrix remodeled by cells cultured in 3D environments. We used this approach to measure the elasticity changes of 3D matrices grown with highly invasive lung cancer cells and cardiac myoblasts, and to delineate the principal mechanism underlying the stiffening of matrices remodeled by these cells. The described approach can be a useful tool in fields investigating and manipulating the mechanotransduction of cells in 3D contexts, and also has potential as a drug-screening platform.
منابع مشابه
Three dimensional shear wave scattering MR elastography
Introduction: Magnetic resonance elastography (MRE) is a non invasive method that combines externally applied vibrations with the motion sensitivity of MRI to measure the shear elasticity soft tissues [1]. In MRE this is traditionally achieved by solving an inverse problem with MRE shear wave images as input data [2]. A complementary approach, which does not require the solution of an inverse p...
متن کاملEvaluation of the diagnostic function of shear wave elastography in the differentiation of malignant and benign breast masses and its relationship with histological factors
Background: Shear wave elastography (SWE) estimates the stiffness of a mass based on the velocity of shear wave propagation by sound waves. Due to higher cell density and angiogenesis, malignant masses have higher stiffness than benign ones. Methods: The present study was a prospective study and was performed on patients who were referred to Ahvaz Golestan Hospital with breast mass during Marc...
متن کاملFeasibility of Two-Dimensional Quantitative Sonoelastographic Imaging
In this paper, a two-dimensional (2D) quantitative sonoelastographic technique for estimating local shear wave speeds from slowly propagating shear wave interference patterns (termed crawling waves) is presented. Homogeneous tissuemimicking phantom results demonstrate the ability of quantitative sonoelastographic imaging to accurately reconstruct the true underlying shear wave speed distributio...
متن کاملLongitudinal shear wave imaging for elasticity mapping using optical coherence elastography.
Shear wave measurements for the determination of tissue elastic properties have been used in clinical diagnosis and soft tissue assessment. A shear wave propagates as a transverse wave where vibration is perpendicular to the wave propagation direction. Previous transverse shear wave measurements could detect the shear modulus in the lateral region of the force; however, they could not provide t...
متن کاملON MAXWELL'S STRESS FUNCTIONS FOR SOLVING THREE DIMENSIONAL ELASTICITY PROBLEMS IN THE THEORY OF ELASTICITY
The governing equations of three dimensional elasticity problems include the six Beltrami-Michell stress compatibility equations, the three differential equations of equilibrium, and the six material constitutive relations; and these are usually solved subject to the boundary conditions. The system of fifteen differential equations is usually difficult to solve, and simplified methods are usual...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره 130 شماره
صفحات -
تاریخ انتشار 2017