On Elastin in Aorta of SHRSP

نویسنده

  • Kozo OKAMOTO
چکیده

Materials and Methods: Male SHRSP and Wistar-Kyoto rats as control were used mainly for this study . Some of the SHRSP were fed with a 50% white fish meal or 50% casein diet by weight from 35 days after birth. After sacrificing these rats at various ages, the thoracic aorta was isolated from each animal and adhering tissues were separated. Five or 6 rats were used for 1 experiment. These aortas were homogenized together in a 0.25M sucrose solution using Virtis homogenizer and nuclear fraction was obtained by centrifuging at 1,000•~g for 10min. According to Partridge's method (Biochem J 61: 11, 1955), an insoluble elastin was prepared from this fraction. The insoluble elastin was weighed and expressed as weight per 1Gm aorta. Five mg of elastin was hydrolyzed in 6 N HCl at 110•Ž for 72 hours and amino acid analysis was performed using a JOEL amino acid analyser.

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تاریخ انتشار 2008