Amino Acids Diets as Model for Investigating Cancer Induced by Acrylamide Produced during Wrong Food Cooking

Starvation could be the cheapest solution for cancer treatment. Alternatively, controlled amino acids diets could be another solution. Starving the body from certain amino acids as well as changing the amount of some used amino acids con-trolled diet is the main idea of this study. In this study, acrylamide was used to induce cancer in the rats. Acrylamide is a cancer causative agent. We utilize it through a diet containing potato. To evaluate the effect of such change in the cancer cells induced by acrylamide in rats, five diets different in their amino acids constituents were designed. Rats were feed on the different amino acids diets to investigate their effect on the cancer induced by the acrylamide. Biochemical analysis for the liver functions represented by GOT, GPT and ALP, assays as well as the amount of the produced billirubin and cholesterol were performed. Additionally the weight and the histo-pathological changes in each of the liver, lung and kidney tissues were investigated. To evaluate the effect of the treatments on the molecular level P53 expression level as a cancer genetic marker were investigated. The results were subjected to logical and statistical analysis. Statistical analyses for the data using LSD were generated. The data obtained from this study prove that the amino acids controlled diets could incapsitate for some extend the development of the cancer cells. In this study, protection against the formation of the cancer is also reported. The main explanation is that such controlled diet might be able to reduce the growth rate of the cancer cells or give suitable time to the cell self-killing mechanism to be complement with the cancer cells replication rate and growing time. Depleting seven amino acids from the diet show the best result (group 7). The study prove that vegetarian, those how fasting or starving are safer and can resist cancer. However they are more susceptible to exogenous source of toxins such as the acrylamide than those who include amino acids in their diets. Controlled amino acids diets show different results according to the amino acids composition. This study recommended use of controlled amino acids to save our health. And more study for the role of the controlled amino acids diets either in cancer protection, prevention or treatment as well as which amino acid(s) should be depleted from which cancer cells should be conducted. List of Abbreviations: ALCRI ;Arid Land Cultivation Research Institute, AmCF; amphophilic cell foci, BCF; basophilic cell foci, CCF clear cell foci, GOT; Glutamic oxaloacetic transaminase, GPT; glutamic pyruvic transaminase, GR; glutathione reductase, LSD; least significant difference, MCF; mixed cell foci, MN; multiple nucleoli, SAM; S-adenosyl-L-methionine, TB; tumor-bearing, TCF; tigroid cell foci, VH; vacuolated hepatocytes. Received: 18 October, 2017; Accepted: 17 January, 2018; Published: 19 February, 2018 *Corresponding author: Gamal M. Hamad, Food Technology Department, Arid Land Cultivation Research Institute, City for Scientific Research and Technological Applications, New Borg AL Arab, Alexandria, Egypt, E-mail : [email protected] Symbiosis www.symbiosisonline.org www.symbiosisonlinepublishing.com Symbiosis Group * Corresponding author email: [email protected] Introduction ‘Cancer’ is a general term applied to a series of malignant diseases characterized by a rapid and uncontrolled formation of abnormal cells which may mass together to form a growth or proliferate throughout the body. Cancer may progress until it causes the death of the organism. Tumors were classified into two main types: benign and malignant. These two types represent the extreme spectrum of tumor growth, while some tumors may exhibit properties of both types, and others may slowly change from one type to another. Benign tumors proliferate locally and are composed of differentiated cells resembling those of the organs’ tissues, where the edge of the tumor is well defined. Malignant tumors are not encapsulated and their edges are ill-defined. Projections of tumor cells extend from the central mass into surrounding tissue. The most common strategy for cancer treatment includes surgery, radiotherapy, chemotherapy, gene therapy, immunotherapy, and treatment with natural plant products. Severe starvation is another tool used by folk. Controlling cancer using complex amino acids imbalance is a rather new strategy aimed to deplete the cancer cell nutritional requirement by introducing synthetic meals to the patient with a defined percentage of each amino acid lead to the death of the cancer cells. In vivo controlling of amino acids using certain enzymes as L-asparaginase is currently used in the treatment of