Membrane topology of microsomal cytochrome P-450: saturation transfer EPR and freeze-fracture electron microscopy studies.

نویسندگان

  • D Schwarz
  • J Pirrwitz
  • H W Meyer
  • M J Coon
  • K Ruckpaul
چکیده

The rotation of cytochrome P-450 LM2 (CYPIIB4) incorporated into large microsomal-like lipid vesicles was investigated by saturation transfer EPR using 15N- and 2H-substituted spin labels. In combination with rotational diffusion, the distribution and size of protein particles in the bilayer were studied by freeze-fracture electron microscopy. The data from both methods suggest an oligomeric and membrane-spanning aggregate for the topology of microsomal cytochrome P-450.

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عنوان ژورنال:
  • Biochemical and biophysical research communications

دوره 171 1  شماره 

صفحات  -

تاریخ انتشار 1990