Multiple isoforms of Pisum trypsin inhibitors result from modification of two primary gene products.
نویسندگان
چکیده
Characterization of Pisum (pea) seed trypsin inhibitors (TI) and their corresponding cDNAs indicates that the pea TI gene family contains two genes. The existence of multiple TI isoforms can be attributed to post-translational modifications of primary gene products. Post-translational processing at the C-terminus during the desiccation stage of seed development results in the appearance of TI isoforms with increased affinity for the target enzyme, trypsin.
منابع مشابه
Human mast cell tryptase isoforms: separation and examination of substrate-specificity differences.
Tryptases are trypsin-like enzymes found in mast cell granules that appear to exist as tetramers. These enzymes are not controlled by blood plasma proteinase inhibitors and only cleave a few physiological substrates in vitro, including high-molecular-mass kininogen (HMMK) and vasoactive intestinal peptide (VIP). Purified human lung mast cell tryptase (HLT) contained two bands of approx. molecul...
متن کاملThe Effect of TiO2-Nanoparticle on the Activity and Stability of Trypsin in Aqueous Medium
Trypsin (E.C.3.4.21.4) is a serine protease commonly used in proteomics for digestion of proteins. In the present study, the effect of nano-TiO2 on the conformation and catalytic activity of trypsin were studied. The thermal denaturation of trypsin has been investigated in the presence and absence of nano-TiO2 over the temperature range (293-373 K) at pH 3.0 and 7.25, using temperature scanning...
متن کاملAmino acid sequences of the two smallest trypsin inhibitors from sponge gourd seeds.
Sponge gourd (Luffa cylindricd) seeds contain several biologically active proteins. Recently, we found that the mature seeds contain three types of trypsin inhibitors having molecular masses of about 13 kDa (type I), 6.5 kDa (type II), and 3.2 kDa (type III). Furthermore, preliminary experiments suggested that the type III one might be a domain unit of these inhibitors. Here, we present the iso...
متن کاملExtrusion of Peas (Pisum sativum L.): Effects on the Apparent Metabolisable Energy and Ileal Nutrient Digestibility of Broilers
Problem statement: The potential feeding values of grain legumes, such as peas (Pisum sativum), are limited because of the presence of anti-nutritional factors. In particular, protease inhibitors are of interest, but these can be readily destroyed by thermal treatments. In the present study, the influence of extrusion on the chemical composition and nutritive value of peas was evaluated. Approa...
متن کاملSmall molecule inhibitors of mesotrypsin from a structure-based docking screen
PRSS3/mesotrypsin is an atypical isoform of trypsin, the upregulation of which has been implicated in promoting tumor progression. To date there are no mesotrypsin-selective pharmacological inhibitors which could serve as tools for deciphering the pathological role of this enzyme, and could potentially form the basis for novel therapeutic strategies targeting mesotrypsin. A virtual screen of th...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- FEBS letters
دوره 360 1 شماره
صفحات -
تاریخ انتشار 1995