Algorithm for the Analysis of Tryptophan Fluorescence Spectra and Their Correlation with Protein Structural Parameters
نویسندگان
چکیده
The fluorescence properties of tryptophan residues are sensitive to the microenvironment of fluorophores in proteins. Therefore, fluorescence characteristics are widely used to study structural transitions in proteins. However, the decoding of the structural information from spectroscopic data is challenging. Here we present a review of approaches developed for the decomposition of multi-component protein tryptophan fluorescence spectra and correlation of these spectral parameters with protein structural properties.
منابع مشابه
Decomposition of protein tryptophan fluorescence spectra into log-normal components. III. Correlation between fluorescence and microenvironment parameters of individual tryptophan residues.
In our previous paper (Reshetnyak, Ya. K., and E. A. Burstein. 2001. Biophys. J. 81:1710-1734) we confirmed the existence of five statistically discrete classes of emitting tryptophan fluorophores in proteins. The differences in fluorescence properties of tryptophan residues of these five classes reflect differences in interactions of excited states of tryptophan fluorophores with their microen...
متن کاملProtein Fluorescence Database
The fluorescence properties of tryptophan residues are sensitive to the microenvironment of tryptophan fluorophores in proteins. Therefore, fluorescence characteristics are widely used to study structural transitions in proteins. We have developed algorithms for the decomposition of multi-component protein fluorescence spectra and the correlation of these spectral parameters with protein struct...
متن کاملمطالعۀ ساختاری برهم کنش داروی ضدسرطان از دستۀ کمپلکس پلاتینی با آلبومین سرم انسانی
Human serum albumin (HSA) is the most abundant protein in blood plasma, which is responsible for 80% of blood pressure; it also acts as a carrier protein for many compounds in the blood such as drugs. In the present study, the interaction and side-effects of a newly-designed anti-cancer compound of isopentyl-glycine1, 10-phenanthroline Platinum nitrate on HSA have been investigated. In this inv...
متن کاملIn vitro study of drug-protein interaction using electronic absorption, fluorescence, and circular dichroism spectroscopy
In the near future, design of a new generation of drugs targeting proteins will be required. Considering the complex bond between the drug and protein, the structure and stability of the target protein should be considered. So far, a series of in vitro investigations have been conducted with the aim of predicting drug-biological medium interactions. In these studies, use of spectroscopic method...
متن کاملDetermining the fluorescence spectrum of a protein.
Fluorescence spectra of proteins are determined chiefly by the polarity of the environment of the tryptophan and tyrosine residues and by their specific interactions. A thorough consideration of fluorescence spectrometers and their calibration is provided along with important information regarding spectrometer cells, buffers and clarification of samples. Protocols are provided for recording flu...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Algorithms
دوره 2 شماره
صفحات -
تاریخ انتشار 2009