Four-way ligation for construction of a mammalian cell-based full-length antibody display library.
نویسندگان
چکیده
A unique four-way ligation strategy was developed for rapid construction of a full-length antibody library. A mammalian expression vector was constructed that contained dual mammalian expression cassettes and sequences recognized by the unique restriction enzymes BsmBI, BstXI, and SfiI. Both full-length light-chain and variable domain of heavy-chain genes were inserted into the vector in one step by four-way ligation, and full-length bivalent antibodies were displayed on mammalian cell surfaces. Using this strategy, only 2 weeks were required to successfully construct high-quality, full-length human antibody libraries.
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عنوان ژورنال:
- Acta biochimica et biophysica Sinica
دوره 43 3 شماره
صفحات -
تاریخ انتشار 2011