Chemical modification of biocatalysts.
Although several powerful methods exist for the redesign of enzyme structure and function these are typically limited to the 20 most abundant proteinogenic amino acids. The use of chemical modification overcomes this limitation to allow virtually unlimited alteration of amino acid sidechain structures. If heterogeneous mixtures of enzyme products are to be avoided, however, the required chemistry should be efficient, selective and compatible with aqueous conditions. Recent advances have been made in the modification of proteinases, aminotransferases and redox enzymes.
Enzymes are able to perform a multitude of chemical and biochemical transformations with efficiencies that are typically unrivalled by chemical catalysts. However, these evolved systems may lack breadth or utility in other non-natural applications. Altering enzyme and protein scaffolds through covalent modification can expand the usefulness of native biocatalysts not only for synthetic applicat...متن کامل
At this short review, different chemical production of 3D graphene biocatalysts and developing of its characters by new substituted for using in enzymatic fuel cells are investigated. Also, the current ways of production of 3D Graphene Biocatalysts, different types of substitutes, the best methods for having the highest efficiency, the physical, chemical and biological characters of new biocata...متن کامل
Because prodrugs and prosupplements (precursors of drugs and dietary supplements that are metabolized within the body to form the corresponding bioactive materials) have recently attracted a great deal of attention, there is a growing need for techniques that are capable of selective chemical modification of functional compounds. We have focused on glycosylation, particularly glucosylation, as ...متن کامل
Over the last two decades, directed evolution has become a staple in protein engineering and ushered in a new era of industrial biocatalysis. Directed evolution has provided the tools to not only improve the activity of known biocatalysts, but also to endow biocatalysts with chemical reactivities not previously encountered in nature. Here we will discuss the recent successes in the quest to enh...متن کامل
Biotransformation of rosamicin antibiotic into 10,11-dihydrorosamicin with enhanced in vitro antibacterial activity against MRSA.
A biotransformation approach using microbes as biocatalysts can be an efficient tool for the targeted modification of existing antibiotic chemical scaffolds to create previously uncharacterized therapeutic agents. By employing a recombinant Streptomyces venezuelae strain as a microbial catalyst, a reduced macrolide, 10,11-dihydrorosamicin, was created from rosamicin macrolide. Its chemical stru...متن کامل