In vivo mapping of nucleosomes using psoralen-DNA crosslinking and primer extension.
نویسندگان
چکیده
By the use of psoralen crosslinking and primer extension, a method was developed which allows the analysis of chromatin structure in vivo. Using a yeast minichromosome, >9 nucleosomes were mapped with a resolution of at least +/-30 bp.
منابع مشابه
Nucleosome positioning, nucleosome spacing and the nucleosome code.
Nucleosome positioning has been the subject of intense study for many years. The properties of micrococcal nuclease, the enzyme central to these studies, are discussed. The various methods used to determine nucleosome positions in vitro and in vivo are reviewed critically. These include the traditional low resolution method of indirect end-labelling, high resolution methods such as primer exten...
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عنوان ژورنال:
- Nucleic acids research
دوره 26 6 شماره
صفحات -
تاریخ انتشار 1998