Assessing genomic DNA contamination of total RNA isolated from kidney cells obtained by Laser Capture Microdissection using the Agilent RNA 6000 Pico assay Application
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چکیده
Total RNA isolated by Laser Capture Microdissection (LCM) from embryonic kidney cells was analyzed for integrity and relative concentration using the Agilent 2100 bioanalyzer and the RNA 6000 Pico assay. An unexpected peak between the 18S and 28S ribosomal RNA peaks was observed and unambiguously proven, by DNase digestion and genomic DNA spike-in experiments, to be caused by genomic DNA contamination. A simple on-column DNase digestion method is suggested to eliminate contaminating genomic DNA from total RNA samples obtained by LCM, fluorescent-activated cell sorting (FACS), or manual dissection. Georgina Caruana Russell McInnes
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تاریخ انتشار 2004