Kinetics of folding of staphylococcal nuclease.
نویسندگان
چکیده
Staplhylococcal nuclease undergoes a reversible structural transition between (p)h3 and 4 which be mesured by changes in tryptoham fluorescence. A stopped-flow spectrofluorometer was used to study the kinetics renaturation of nuclease from the acidified form on neutralization, the refolding is fast and the data can be described as a sequence of two first-order processes with half times of about 55 and 350 milliseconds, respectively.
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عنوان ژورنال:
- Science
دوره 167 3919 شماره
صفحات -
تاریخ انتشار 1970