Temperature impairment of telomere function 1 A naturally thermolabile activity compromises genetic analysis of telomere function in Saccharomyces cerevisiae
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چکیده
* Salk Institute for Biological Studies, La Jolla, CA 92037-1099, USA § Division of Biological Sciences, University of California San Diego, La Jolla, CA 92093-0130, USA ‡ Department of Molecular and Human Genetics, Baylor College of Medicine, Houston TX 77030, USA 1 current address: Sanford-Burnham Medical Research Institute, La Jolla, CA 92037, USA Genetics: Published Articles Ahead of Print, published on February 29, 2012 as 10.1534/genetics.111.137869
منابع مشابه
A naturally thermolabile activity compromises genetic analysis of telomere function in Saccharomyces cerevisiae.
The core assumption driving the use of conditional loss-of-function reagents such as temperature-sensitive mutations is that the resulting phenotype(s) are solely due to depletion of the mutant protein under nonpermissive conditions. However, prior published data, combined with observations presented here, challenge the generality of this assumption at least for telomere biology: for both wild-...
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In Saccharomyces cerevisiae, Cdc13 binds telomeric DNA to recruit telomerase and to "cap" chromosome ends. In temperature-sensitive cdc13-1 mutants telomeric DNA is degraded and cell-cycle progression is inhibited. To identify novel proteins and pathways that cap telomeres, or that respond to uncapped telomeres, we combined cdc13-1 with the yeast gene deletion collection and used high-throughpu...
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In budding yeast Saccharomyces cerevisiae, telomere length maintenance involves a complicated network as more than 280 telomere maintenance genes have been identified in the nonessential gene deletion mutant set. As a supplement, we identified additional 29 telomere maintenance genes, which were previously taken as essential genes. In this study, we report a novel function of Sua5p in telomere ...
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Previous work in budding yeast has indicated that telomeres are protected, at least in part, from the action of Exo1, which degrades the C-rich strand of partially uncapped telomeres. To explore this further, we examined the consequences of Exo1-mediated activity in strains that lacked Ku, telomerase, or both. Loss of Exo1 partially rescued the telomere length defect in a yku80delta strain, dem...
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تاریخ انتشار 2012