Many Methods, Many Microbes: Methodological Diversity and Standardization in the Deep Subseafloor Biosphere
نویسندگان
چکیده
Standardization is widely assumed to be important to advance science. This assumption is typically embedded in initiatives to devise infrastructure and policies to support scientific work. This paper examines a movement comprising scientists advocating methods standardization in an emerging scientific domain, the deep subseafloor biosphere. This movement is not primarily motivated by the usual rationales for standardization, but instead by the aim of intervening in the politics of an infrastructure upon which the domain depends, scientific ocean drilling cruises. This infrastructure is shared and contested with other domains, and this movement regards standardization as a critical step in reconfiguring the infrastructure to secure a greater share of resources for the deep subseafloor biosphere. This movement encounters two tensions. One tension is between the perceived benefits of standardization vs. methodological diversity. Another tension is between perceived benefits for the domain vs. a lack of incentives for individuals to perform necessary standardization work.
منابع مشابه
Phylogenetic Diversity of aprA Genes in Subseafloor Sediments on the Northwestern Pacific Margin off Japan
Markedly diverse sequences of the adenosine-5'-phosphosulfate reductase alpha subunit gene (aprA), which encodes a key enzyme in microbial sulfate reduction and sulfur oxidation, were detected in subseafloor sediments on the northwestern Pacific off Japan. The aprA gene sequences were grouped into 135 operational taxonomic units (90% sequence identity), including genes related to putative sulfu...
متن کاملShip space to database: emerging infrastructures for studies of the deep subseafloor biosphere
Background. An increasing array of scientific fields face a ‘‘data deluge.’’ However, in many fields data are scarce, with implications for their epistemic status and ability to command funding. Consequently, they often attempt to develop infrastructure for data production, management, curation, and circulation. A component of a knowledge infrastructure may serve one or more scientific domains....
متن کاملBiogeographical distribution and diversity of microbes in methane hydrate-bearing deep marine sediments on the Pacific Ocean Margin.
The deep subseafloor biosphere is among the least-understood habitats on Earth, even though the huge microbial biomass therein plays an important role for potential long-term controls on global biogeochemical cycles. We report here the vertical and geographical distribution of microbes and their phylogenetic diversities in deeply buried marine sediments of the Pacific Ocean Margins. During the ...
متن کاملAcetogenesis in the Energy-Starved Deep Biosphere – A Paradox?
Under anoxic conditions in sediments, acetogens are often thought to be outcompeted by microorganisms performing energetically more favorable metabolic pathways, such as sulfate reduction or methanogenesis. Recent evidence from deep subseafloor sediments suggesting acetogenesis in the presence of sulfate reduction and methanogenesis has called this notion into question, however. Here I argue th...
متن کاملTurnover of microbial lipids in the deep biosphere and growth of benthic archaeal populations.
Deep subseafloor sediments host a microbial biosphere with unknown impact on global biogeochemical cycles. This study tests previous evidence based on microbial intact polar lipids (IPLs) as proxies of live biomass, suggesting that Archaea dominate the marine sedimentary biosphere. We devised a sensitive radiotracer assay to measure the decay rate of ([(14)C]glucosyl)-diphytanylglyceroldiether ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2016