Properties and immobilization of urease from leaves of Chenopodium album ( C 3 )
In Chenopodium album, leaf excision and light both increase urease (EC. 126.96.36.199) activity. Dithiothreitol (DTT), reduced glutathione (GSH), cysteine and diazoinedicarboxylic acid bis(N,N-dimethylamide) (diamide) activated the crude enzyme. In contrast, crude urease was inhibited by phenylmethylsulphonyl fluoride (PMSF) and Na-p -tosyl-L-lysine chloromethyl ketone HCl (TLCK), suggesting the presence of serine and histidine residues in the active site. The enzyme is Ca dependent for its activity and exogenous calmodulin (CaM) did not stimulate it. However the enzyme is strongly inhibited by CaM antagonist fluphenazine, indicating the presence of a Ca-like domain. EGTA, LaCl 3 and tetraacetic acid, 3,4,5,-trimethoxybenzoic acid 8-(diethyl-amino)-octyl ester (TMB-8) inhibited urease activity in vivo, and the inhibition was restored by exogenous Ca. Urease was immobilized in gelatin by covalent cross-linking with formaldehyde as organic hardener. The results indicated enhanced resistance to thermal denaturation, increased temperature optima (from 30°C to 40°C), and a rapid rate of substrate saturation were achieved after immobilization. The free urease showed remarkable loss of activity in the presence of sodium dodecyl sulphate, sodium deoxycholate, sodium taurocholate, Triton X-100, and Tween 80. The free enzyme lost 68%, 75% and 81% of its activity in the presence of 5,5 ́-dithiobis-(2-nitrobenzoic acid) (NBS 2 ), p-hydroxymercuribenzoate (PHMB) and phenylmercuric acetate (PMA) as thiol reagents. However, the immobilized enzyme was not affected significantly by these compounds. By increasing the incubation time, the activity of immobilized enzyme decreased faster than that of the free one.
Fractionation of crude petroleum ether extract of the leaves of Chenopodium album Linn lead to the isolation of β-sitosterol (1), lupeol (2) and 3 hydroxy nonadecyl henicosanoate (3). Their structures were elucidated by spectroscopic methods such as UV, IR, NMR and LCMS. Compound 2 and 3 were isolated for the first time from this plant.متن کامل
Recent years have instance that there is a invigoration of interest in drug discovery from medicinal plants for the support of health in all parts of the world . This study was designed to examine the in vitro antimicrobial activities of the flowers and leaves methanolic and ethanolic extracts of Chenopodium album L. Chenopodium album Linn. flowers and leaves were collected from East Esfahan, I...متن کامل
Physico-chemical and textural property of starch isolated from Chenopodium (Chenopodium album) grains
The aim of current study was to isolate and evaluate the starch from Chenopodium album (LP) and Chenopodium album (LS) varieties for its physicochemical, particle size analysis, and textural properties. The starch isolated from C. album (LP) and C. album (LS) showed yield values of 37.59 and 47.30%, while amylose content of the mentioned varieties was found to be 16.75 and 19.11%, respectively....متن کامل
Background & Objectives: In recent decades, rate of infections caused by opportunistic fungi, such as Candida albicans, has increased dramatically. Treatment of fungal diseases is associated with limitations such as rare and expensive antifungal drugs and their side effects, drug resistance and reduced susceptibility of fungi to drugs. Therefore, researchers have been concerned with finding new...متن کامل
Background: The incidence of allergic and asthmatic diseases has been continuously increased in both industrial and developing countries. Extracts from various known allergens are used for the diagnostic and therapeutic purposes. Objective: To investigate the effects of an extract prepared from Chenopodium album (Ch.A.) pollen to induce allergic asthma in BALB/C mice. Methods: BALB/C mice were ...متن کامل