Metabolism of Terbufos in Rat Liver

Metabolism of Terbufos in Rat Liver: JinTong LI, et al. Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing, China, 100850—Biotransformation of terbufos in rat liver revealed four metabolites in the effluent prepared with a C18 cartridge after the rat liver was perfused for one hour in situ. Analysing the spectrogram of GC-IR and GC-MS, metabolite IV appeared to be an oxidative desulfuration product of terbufos with the formula C9H21O3PS2, the recovery of which in the effluent was 2.13%; metabolite I appeared to be an hydrolysate of metabolite IV with the formula C5H13O3PS, the recovery of which was 0.13%; metabolite II appeared to be an hydrolysate of terbufos with the formula C5H13O2PS2, the recovery of which was 2.65%; metabolite III appeared to be a methylate of metabolite II, with the formula C 6 H 15 O 2 PS 2 , the recovery of which was 1.42%. Relatively the recovery of terbufos was 40.8%. These results were in accord with the regular metabolic pattern in vivo of phosphorothioates with a thioether group. (J Occup Health 1999; 41: 62–68)