Biosynthesis in Aerobacter aerogenes

Enzymes essential for the biosynthesis of nucleic acid guanine; xanthosine 5'-phosphate aminase of Aerobacter aerogenes.

Earlier studies have shown1 X5’P to be an obligatory intermediate in the biosynthesis of nucleic acid guanine in Aerobacter aerogenes and related organisms (24). The present paper describes the isolation of X5’P aminase, the enzyme responsible for the conversion of X5’P to G5’P, from extracts of A. aerogenes, and discusses the mechanism of this reaction and its role in purine metabolism. A simi...

The biosynthesis of capsular polysaccharide in Aerobacter aerogenes.

Effect of mixtures of substrates on the biosynthesis of inducible enzymes in Aerobacter aerogenes.

Previous investigations in this laboratory have dealt with the striking effect of glucose on the induced biosynthesis of three enzyme systems in Aerobacter aerogenes (Magasanik, 1955; Neidhardt and Magasanik, 1956a). During the course of these studies it was found that glucose is not the only substance whose inclusion in a growth medium restricts induced enzyme biosynthesis, although it is uniq...

The metabolism of purines in Aerobacter aerogenes: a study of purineless mutants.

Amino acid deficient bacterial mutants have been of great value in the elucidation of the biosynthesis and the metabolic relationships of amino acids (Davis, 1952). Similarly, purineless and pyrimidineless mutants should be of value for the study of the metabolism of the components of nucleic acids. However, most purineless mutants isolated have been found to be nonexacting in their requirement...

Cystathionine as a precursor of methionine in Escherichia coli and Aerobacter aerogenes.

Balish, Edward (Argonne National Laboratory, Argonne, Ill.), and Stanley K. Shapiro. Cystathionine as a precursor of methionine in Escherichai coli and Aerobacter aerogenes. J. Bacteriol. 92:1331-1336. 1966.-Cystathionine has been shown to be a precursor of methionine biosynthesis in Escherichia coli and Aerobacter aerogenes. A double enzyme assay was developed to show the formation of homocyst...