Prediction and classification of the modes of genotoxic actions using bacterial biosensors and cell array chip

in dry chamber for 2 days 0.7 mL of the cell suspensionwas inoculated into a vessel of model soil and kept at 30 °C. N-hexadecane was quantified with FID-GC after extraction with chloroform. As the results, it was found that the degradation rate reached plateau over the hexadecane concentration of 1.1 mg (g-soil). This suggests that the rate-limiting step in the degradation of hexadecane in soil is not the transportation of the hydrophobic substance through aqueous phase but the reactions that degrade the substance. Thus, we tried to clarify the rate-limiting reaction in the metabolic degradation pathway of hexadecane and on the other hand to prolong the degradation reaction of Y. lipolytica.