Fast and convenient 5-hydroxymethylcytosine enrichment workflow for next-generation sequencing
نویسندگان
چکیده
5-hydroxymethylcytosine (5-hmC) is an extensively studied DNA epigenetic modification. Here we present a novel tool which combined with next generation sequencing offers new ways of analyzing 5-hmC at the genomic level. We demonstrate that the Thermo ScientificTM EpiJETTM 5-hmC Enrichment Kit is highly specific for different DNA samples containing 5-hmC modifications. Our data shows that this tool can be used for locusspecific and whole genome–specific analysis resulting in 5-hmC distribution patterns across different genomes.
منابع مشابه
5-Hydroxymethylcytosine (5-hmC) Specific Enrichment.
5-Hydroxymethylcytosine (5-hmC) is a newly discovered DNA modification in mammalian genomes. This protocol is to be a highly efficient and selective chemical approach to label and capture 5-hmC, taking advantage of a bacteriophage enzyme that adds a glucose moiety to 5-hmC specifically, which could in turn be used for high-throughput mapping via next-generation sequencing.
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تاریخ انتشار 2014